diff --git a/integration-test/src/test/java/org/apache/iotdb/ainode/it/AINodeCallInferenceIT.java b/integration-test/src/test/java/org/apache/iotdb/ainode/it/AINodeCallInferenceIT.java
index 9bf0927bb622..852827aa8ab9 100644
--- a/integration-test/src/test/java/org/apache/iotdb/ainode/it/AINodeCallInferenceIT.java
+++ b/integration-test/src/test/java/org/apache/iotdb/ainode/it/AINodeCallInferenceIT.java
@@ -40,6 +40,7 @@
 
 import static org.apache.iotdb.ainode.utils.AINodeTestUtils.BUILTIN_MODEL_MAP;
 import static org.apache.iotdb.ainode.utils.AINodeTestUtils.checkHeader;
+import static org.apache.iotdb.ainode.utils.AINodeTestUtils.errorTest;
 import static org.apache.iotdb.ainode.utils.AINodeTestUtils.prepareDataInTree;
 
 @RunWith(IoTDBTestRunner.class)
@@ -72,6 +73,7 @@ public void callInferenceTest() throws SQLException {
           Statement statement = connection.createStatement()) {
         callInferenceTest(statement, modelInfo);
         callInferenceByDefaultTest(statement, modelInfo);
+        callInferenceErrorTest(statement, modelInfo);
       }
     }
   }
@@ -118,4 +120,16 @@ public static void callInferenceByDefaultTest(
       }
     }
   }
+
+  public static void callInferenceErrorTest(
+      Statement statement, AINodeTestUtils.FakeModelInfo modelInfo) {
+    String multiVariateSQL =
+        String.format(
+            "CALL INFERENCE(%s, \"SELECT s0,s1 FROM root.AI LIMIT 128\", generateTime=true, outputLength=10)",
+            modelInfo.getModelId());
+    errorTest(
+        statement,
+        multiVariateSQL,
+        "701: Call inference function should not contain more than one input column, found [2] input columns.");
+  }
 }
diff --git a/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/queryengine/execution/operator/process/ai/InferenceOperator.java b/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/queryengine/execution/operator/process/ai/InferenceOperator.java
index 8bf9f5ead14b..d39d81aff5be 100644
--- a/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/queryengine/execution/operator/process/ai/InferenceOperator.java
+++ b/iotdb-core/datanode/src/main/java/org/apache/iotdb/db/queryengine/execution/operator/process/ai/InferenceOperator.java
@@ -24,6 +24,7 @@
 import org.apache.iotdb.commons.client.exception.ClientManagerException;
 import org.apache.iotdb.db.exception.ainode.AINodeConnectionException;
 import org.apache.iotdb.db.exception.runtime.ModelInferenceProcessException;
+import org.apache.iotdb.db.exception.sql.SemanticException;
 import org.apache.iotdb.db.protocol.client.an.AINodeClient;
 import org.apache.iotdb.db.protocol.client.an.AINodeClientManager;
 import org.apache.iotdb.db.queryengine.execution.MemoryEstimationHelper;
@@ -225,6 +226,12 @@ private void appendTsBlockToBuilder(TsBlock inputTsBlock) {
         maxTimestamp = Math.max(maxTimestamp, timestamp);
       }
       timeColumnBuilder.writeLong(timestamp);
+      if (inputTsBlock.getValueColumnCount() > 1) {
+        throw new SemanticException(
+            String.format(
+                "Call inference function should not contain more than one input column, found [%d] input columns.",
+                inputTsBlock.getValueColumnCount()));
+      }
       for (int columnIndex = 0; columnIndex < inputTsBlock.getValueColumnCount(); columnIndex++) {
         columnBuilders[columnIndexes[columnIndex]].write(inputTsBlock.getColumn(columnIndex), i);
       }